Skip to main content
Addgene

SELENOM SXXS
(Plasmid #86580)

Print

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 86580 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
Add to Cart

* Log in to view industry pricing.

Backbone

  • Vector backbone
    pMAL-c5X
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SELENOM
  • Species
    H. sapiens (human)
  • Mutation
    Sigal peptide was deleted; SXXS
  • Entrez Gene
    SELENOM (a.k.a. SELM, SEPM)
  • Promoter Tac
  • Tag / Fusion Protein
    • His6-MBP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer MBP-F
  • 3′ sequencing primer TGTCCTACTCAGGAGAGCGTTCAC
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SELENOM SXXS was a gift from Sharon Rozovsky (Addgene plasmid # 86580 ; http://n2t.net/addgene:86580 ; RRID:Addgene_86580)

  • For your References section:

    Utilizing Selenocysteine for Expressed Protein Ligation and Bioconjugations. Liu J, Chen Q, Rozovsky S. J Am Chem Soc. 2017 Feb 10. doi: 10.1021/jacs.6b10991. 10.1021/jacs.6b10991 PubMed 28186733
Related items: