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Addgene

p4479 GST-E6AP C833A
(Plasmid #8656)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8656 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGEX-2T
  • Backbone size w/o insert (bp) 4948
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    E6-AP
  • Species
    H. sapiens (human)
  • Mutation
    C833A; active site Cys mutated to encode Ala
  • Entrez Gene
    UBE3A (a.k.a. ANCR, AS, E6-AP, EPVE6AP, HPVE6A, PIX1)
  • Tag / Fusion Protein
    • GST

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (destroyed during cloning)
  • 5′ sequencing primer pGEX-5'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See author's map from plasmid 8655 (howley plasmid 4028) for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p4479 GST-E6AP C833A was a gift from Peter Howley (Addgene plasmid # 8656)

  • For your References section:

    Regulation of the Src family tyrosine kinase Blk through E6AP-mediated ubiquitination. Oda H, Kumar S, Howley PM. Proc Natl Acad Sci U S A 1999 Aug 17;96(17):9557-62. 10.1073/pnas.96.17.9557 PubMed 10449731
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