p4028 GST-E6AP
(Plasmid #8655)

• Depositing Lab: Peter Howley
• Publication: Oda et al Proc Natl Acad Sci U S A 1999 Aug 17;96(17):9557-62.

Backbone

• Vector backbone: pGEX-2T
• Backbone size w/o insert (bp): 4948
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: E6-AP
• Species: H. sapiens (human)
• Insert Size (bp): 2500
• Entrez Gene: UBE3A (a.k.a. ANCR, AS, E6-AP, EPVE6AP, HPVE6A, PIX1)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (destroyed during cloning)
• 5′ sequencing primer: pGEX-5'

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See author's map. E6-AP is missing the first 22 amino acids of isoform 1 (protein starts with MEACT NEFCA).

There is also an N->K mutation 20 amino acids downstream from the beginning of the insert. This mutation has no known functional ramifications.