Skip to main content
Addgene

p3992 pET ubcH5
(Plasmid #8652)

Print

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8652 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET-3a
  • Backbone size w/o insert (bp) 4640
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    UbcH5
  • Species
    H. sapiens (human)
  • Entrez Gene
    UBE2D1 (a.k.a. E2(17)KB1, SFT, UBC4/5, UBCH5, UBCH5A)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See author's map.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3992 pET ubcH5 was a gift from Peter Howley (Addgene plasmid # 8652 ; http://n2t.net/addgene:8652 ; RRID:Addgene_8652)

  • For your References section:

    Identification of a human ubiquitin-conjugating enzyme that mediates the E6-AP-dependent ubiquitination of p53. Scheffner M, Huibregtse JM, Howley PM. Proc Natl Acad Sci U S A 1994 Sep 13;91(19):8797-801. 10.1073/pnas.91.19.8797 PubMed 8090726
Related items:
Commonly requested with: