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Addgene

p3991 pET ubcH9
(Plasmid #8651)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8651 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET23a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 3700
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    UbcH9
  • Alt name
    UBC9
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    600
  • Entrez Gene
    UBE2I (a.k.a. C358B7.1, P18, UBC9)
  • Tag / Fusion Protein
    • HA (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See author's map.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3991 pET ubcH9 was a gift from Peter Howley (Addgene plasmid # 8651 ; http://n2t.net/addgene:8651 ; RRID:Addgene_8651)

  • For your References section:

    Physical interaction between specific E2 and Hect E3 enzymes determines functional cooperativity. Kumar S, Kao WH, Howley PM. J Biol Chem 1997 May 23;272(21):13548-54. 10.1074/jbc.272.21.13548 PubMed 9153201
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