CRAF_S259A_pcDNA3.1
(Plasmid #86505)

• Purpose: Expresses CRAF_S259A in mammalian cells
• Depositing Lab: Dustin Maly
• Publication: Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038/nchembio.2244.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 5574
• Total vector size (bp): 7521
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CRAF
• Alt name: RAF1
• Alt name: Raf-1 proto-oncogene, serine/threonine kinase
• Alt name: C-RAF
• Species: H. sapiens (human)
• Insert Size (bp): 1977
• Mutation: S259A
• GenBank ID: NM_002880.3
• Entrez Gene: RAF1 (a.k.a. CMD1NN, CRAF, NS5, Raf-1, c-Raf)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV_F
• 3′ sequencing primer: BGHR

Resource Information

• Supplemental Documents:
  • CRAF_S259A_pcDNA3.1.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry