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GFP-P2A-CIAR_pLenti_CMV_Puro
(Plasmid #86503)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86503 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti CMV GFP Puro (Addgene 17448)
  • Backbone manufacturer
    Eric Campeau
  • Backbone size w/o insert (bp) 7888
  • Total vector size (bp) 10885
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP-P2A-CIAR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2997
  • Mutation
    T968L in SOScat
  • Entrez Gene
    SOS1 (a.k.a. GF1, GGF1, GINGF, HGF, NS4, SOS-1)
  • Promoter CMV

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-P2A-CIAR_pLenti_CMV_Puro was a gift from Dustin Maly (Addgene plasmid # 86503 ; http://n2t.net/addgene:86503 ; RRID:Addgene_86503)

  • For your References section:

    A computationally engineered RAS rheostat reveals RAS-ERK signaling dynamics. Rose JC, Huang PS, Camp ND, Ye J, Leidal AM, Goreshnik I, Trevillian BM, Dickinson MS, Cunningham-Bryant D, Debnath J, Baker D, Wolf-Yadlin A, Maly DJ. Nat Chem Biol. 2016 Nov 21. doi: 10.1038/nchembio.2244. 10.1038/nchembio.2244 PubMed 27870838
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