CIAR_pcDNA5/FRT/TO
(Plasmid #86502)

• Purpose: Expresses CIAR in mammalian cells. Used to generate Flp-In stables.
• Depositing Lab: Dustin Maly
• Publication: Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038/nchembio.2244.

Backbone

• Vector backbone: pcDNA5/FRT/TO
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5137
• Total vector size (bp): 7339
• Vector type: Mammalian Expression ; FRT
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CIAR
• Alt name: S.17.3
• Alt name: Chemically Inducible Activator of RAS
• Species: H. sapiens (human)
• Insert Size (bp): 2202
• Mutation: T968L in SOScat
• Entrez Gene: SOS1 (a.k.a. GF1, GGF1, GINGF, HGF, NS4, SOS-1)
• Promoter: CMV (tet operator)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV_F
• 3′ sequencing primer: BGHR

Resource Information

• Supplemental Documents:
  • CIAR_pcDNA5.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry