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pT3-EF1aH NICD1
(Plasmid #86500)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86500 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pT3-EF1aH
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Notch1 (C-term)
  • Alt name
    Notch 1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2385
  • Mutation
    Cytoplasmic domain only (AA 1753-2531)
  • GenBank ID
    NM_008714.3
  • Entrez Gene
    Notch1 (a.k.a. 9930111A19Rik, Mis6, N1, Tan1, lin-12)
  • Promoter EF1a
  • Tag / Fusion Protein
    • Myc (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT3-EF1aH NICD1 was a gift from Xin Chen (Addgene plasmid # 86500 ; http://n2t.net/addgene:86500 ; RRID:Addgene_86500)

  • For your References section:

    Cholangiocarcinomas can originate from hepatocytes in mice. Fan B, Malato Y, Calvisi DF, Naqvi S, Razumilava N, Ribback S, Gores GJ, Dombrowski F, Evert M, Chen X, Willenbring H. J Clin Invest. 2012 Aug 1;122(8):2911-5. doi: 10.1172/JCI63212. Epub 2012 Jul 17. 10.1172/JCI63212 PubMed 22797301
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