pT3-EF1aH N90-beta-catenin
(Plasmid #86499)

• Purpose: Mammalian expression of Myc tagged N90 beta-catenin
• Depositing Lab: Xin Chen
• Publication: Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;104(37):14771-6. Epub 2007 Sep 4.

Backbone

• Vector backbone: pT3-EF1aH
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: beta-catenin
• Alt name: b-catenin
• Alt name: CTNNB1
• Species: H. sapiens (human)
• Insert Size (bp): 2121
• Mutation: Deletion of amino acids 1-90; constitutively active
• Entrez Gene: CTNNB1 (a.k.a. CTNNB, EVR7, MRD19, NEDSDV, armadillo)
• Promoter: EF1a
• Tag / Fusion Protein:
  • Myc (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: EF1a-F

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry