MoFGFR31c-ires neo
(Plasmid
#86496)
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Purposemamalian cell expression of FGFR3-FGFR1 chimera
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 86496 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneBluescript
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFgfrR3-1 IIIc 15C7
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SpeciesM. musculus (mouse)
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MutationFGFR3-FGFR1 chimera
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Entrez GeneFgfr1 (a.k.a. Eask, FGFR-I, FLG, Fgfr-1, Flt-2, Fr1, Hspy, MFR, bFGF-R-1, c-fgr)
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Entrez GeneFgfr3 (a.k.a. CD333, FR3, Fgfr-, Fgfr-3, Flg-2, HBGF, HBGFR, Mfr3, sa, sam3)
- Promoter Moloney murine leukemia virus LTR
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13-F20
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MoFGFR31c-ires neo was a gift from David Ornitz (Addgene plasmid # 86496 ; http://n2t.net/addgene:86496 ; RRID:Addgene_86496) -
For your References section:
Receptor specificity of the fibroblast growth factor family. Ornitz DM, Xu J, Colvin JS, McEwen DG, MacArthur CA, Coulier F, Gao G, Goldfarb M. J Biol Chem. 1996 Jun 21;271(25):15292-7. PubMed 8663044