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Addgene

pYES2-HTH-HHT2
(Plasmid #86482)

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Item Catalog # Description Quantity Price (USD)
Plasmid 86482 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pYES2-HTH
  • Backbone manufacturer
    Akash Gunjan, Addgene Plasmid 86470
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    HHT2
  • Alt name
    Histone H3
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    HHT2 (a.k.a. YNL031C)
  • Promoter GAL1
  • Tag / Fusion Protein
    • HIS10-TEV-HA (HTH) (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer GAL1
  • 3′ sequencing primer CYC1
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYES2-HTH-HHT2 was a gift from Akash Gunjan (Addgene plasmid # 86482 ; http://n2t.net/addgene:86482 ; RRID:Addgene_86482)

  • For your References section:

    Histone levels are regulated by phosphorylation and ubiquitylation-dependent proteolysis. Singh RK, Kabbaj MH, Paik J, Gunjan A. Nat Cell Biol. 2009 Aug;11(8):925-33. doi: 10.1038/ncb1903. Epub 2009 Jul 5. 10.1038/ncb1903 PubMed 19578373
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