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Addgene

pCAM105
(Plasmid #86479)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86479 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    CDF-Duet
  • Backbone manufacturer
    Novagen
  • Modifications to backbone
    tet repressor and promoter have been cloned to replace the t7 promoter.
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    p9-FLAG-FMO
  • Species
    Synthetic; Bacteriophage phi6
  • Promoter pTet
  • Tags / Fusion Proteins
    • FLAG (C terminal on insert)
    • FMO (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CATAATTGTTGCAATGAAACGCGGTG
  • 3′ sequencing primer CAGCCAAGCTTGCATGCC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAM105 was a gift from Pamela Silver (Addgene plasmid # 86479 ; http://n2t.net/addgene:86479 ; RRID:Addgene_86479)

  • For your References section:

    Synthetic Lipid-Containing Scaffolds Enhance Production by Colocalizing Enzymes. Myhrvold C, Polka JK, Silver PA. ACS Synth Biol. 2016 Aug 3. 10.1021/acssynbio.6b00141 PubMed 27487319
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