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Purpose(Empty Backbone) Yeast expression vector containing HIS10-TEV-HA (HTH) epitope tag downstream of the GAL1 promoter
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 86470 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepYES2
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Backbone manufacturerInvitrogen
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Modifications to backboneadded HIS10-TEV-HA (HTH) epitope tag downstream of the GAL1 promoter between the HindIII and KpnI restriction sites
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Vector typeYeast Expression
- Promoter GAL1
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Selectable markersURA3
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Tag
/ Fusion Protein
- HIS10-TEV-HA (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer GAL1
- 3′ sequencing primer CYC1 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYES2-HTH was a gift from Akash Gunjan (Addgene plasmid # 86470 ; http://n2t.net/addgene:86470 ; RRID:Addgene_86470) -
For your References section:
Histone levels are regulated by phosphorylation and ubiquitylation-dependent proteolysis. Singh RK, Kabbaj MH, Paik J, Gunjan A. Nat Cell Biol. 2009 Aug;11(8):925-33. doi: 10.1038/ncb1903. Epub 2009 Jul 5. 10.1038/ncb1903 PubMed 19578373
