pPotef-cdc3CB16-NatR
(Plasmid #86465)

• Purpose: Expresses an N-terminal Cdc3-mCherry fusion under the control of the constitutive Potef promotor. 16boxB stem-loops are integrated into the 3'UTR to visualize the mRNA.
• Depositing Lab: Michael Feldbrügge
• Publication: Baumann et al EMBO Rep. 2014 Jan;15(1):94-102. doi: 10.1002/embr.201338037. Epub 2013 Dec 15.

Backbone

• Vector backbone: pUC57
• Total vector size (bp): 12881
• Vector type: Bacterial Expression ; Ustilago maydis Expression
• Selectable markers: Nourseothricin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cdc3
• Alt name: Septin
• Species: U. maydis
• GenBank ID: umag10503
• Promoter: Potef
• Tag / Fusion Protein:
  • mCherry (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsrgI (unknown if destroyed)
• 3′ cloning site: SacII (unknown if destroyed)
• 5′ sequencing primer: TGCGAGGGTAGAGTGTGG
• 3′ sequencing primer: CGCCTCTGCACATCTCTG

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry