pBac[3xP3-EGFP;Tc'hsp5'-Gal4Delta-3'UTR]
(Plasmid
#86449)
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PurposepBAc transposon with transformation marker for Tribolium transgenesis; Drives EGFP expression in the eyes; contains Gal4_ driven by Tribolium heatshock promoter; (Transformation plasmid)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 86449 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBac
- Backbone size w/o insert (bp) 5500
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name3xP3-EGFP;Tc'hsp5'-Gal4Delta-3'UTR
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
cassette exchange by FseI or AscI; Literature: Horn et al. 2000 doi:10.1007/s004270000110; Berghammer et al. 1999 DOI:10.1038/46463
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBac[3xP3-EGFP;Tc'hsp5'-Gal4Delta-3'UTR] was a gift from Gregor Bucher (Addgene plasmid # 86449 ; http://n2t.net/addgene:86449 ; RRID:Addgene_86449)
