pmCherry-GFP
(Plasmid #86441)

• Purpose: visualization and quantifying the out-of-frame mutations from genome editing
• Depositing Lab: Yun-Bo Shi
• Publication: Fu et al Sci Rep. 2016 Oct 17;6:35488. doi: 10.1038/srep35488.

Backbone

• Vector backbone: pUC19
• Backbone manufacturer: Thermofischer
• Backbone size w/o insert (bp): 2687
• Total vector size (bp): 3963
• Vector type: Color visualization in bacteria

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mCherry-MCS-sfGFP fusion gene
• Insert Size (bp): 1530
• Promoter: Plac
• Tag / Fusion Protein:
  • mCherry-sfGFP

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: NdeI (destroyed during cloning)
• 5′ sequencing primer: M13R

Resource Information

• Supplemental Documents:
  • pmCherry-GFP.gbk
• A portion of this plasmid was derived from a plasmid made by: mCherry was PCR-amplified from Addgene clone#59995; sfGFP gene was synthesized based on sequences of GenBank # HQ873313. sfGFP gene was synthesized based on sequences of GenBank # HQ873313.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The whole plasmid sequences were compiled based on vector sequences information. It was not confirmed by DNA sequencing.