Skip to main content
Addgene

pLacZa-GFP
(Plasmid #86440)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86440 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pUC19
  • Backbone manufacturer
    Thermofischer
  • Backbone size w/o insert (bp) 2687
  • Total vector size (bp) 3504
  • Vector type
    Color visualization in bacteria

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LacZalpha-MCS-sfGFP fusion gene
  • Insert Size (bp)
    1072
  • Promoter Plac
  • Tag / Fusion Protein
    • LacZa-sfGFP

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site NdeI (destroyed during cloning)
  • 5′ sequencing primer M13R
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    LacZa was PCR amplified from pUC19 of Thermofischer and sfGFP gene was synthesized based on sequences of GenBank # HQ873313. sfGFP gene was synthesized based on sequences of GenBank # HQ873313.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The whole plasmid sequences were compiled based on vector sequences information. It was not confirmed by DNA sequencing.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLacZa-GFP was a gift from Yun-Bo Shi (Addgene plasmid # 86440 ; http://n2t.net/addgene:86440 ; RRID:Addgene_86440)

  • For your References section:

    A simple and efficient method to visualize and quantify the efficiency of chromosomal mutations from genome editing. Fu L, Wen L, Luu N, Shi YB. Sci Rep. 2016 Oct 17;6:35488. doi: 10.1038/srep35488. 10.1038/srep35488 PubMed 27748423
Related items: