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PurposeSingle-construct blue light optogenetic Ras activator for use in Drosophila
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 86439 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepTIGER
- Backbone size w/o insert (bp) 10100
- Total vector size (bp) 13348
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameOptoSOS
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SpeciesH. sapiens (human), Synthetic
- Promoter Gal4-UAS
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Tag
/ Fusion Protein
- tagRFP (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTiger-OptoSOS was a gift from Jared Toettcher (Addgene plasmid # 86439 ; http://n2t.net/addgene:86439 ; RRID:Addgene_86439) -
For your References section:
The Spatiotemporal Limits of Developmental Erk Signaling. Johnson HE, Goyal Y, Pannucci NL, Schupbach T, Shvartsman SY, Toettcher JE. Dev Cell. 2017 Jan 23;40(2):185-192. doi: 10.1016/j.devcel.2016.12.002. 10.1016/j.devcel.2016.12.002 PubMed 28118601
Map uploaded by the depositor.
