Skip to main content
Addgene

pGL4.10-Rnd3 promoter [-1759 +248]
(Plasmid #86438)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86438 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pGL4.10
  • Backbone manufacturer
    Promega
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Rnd3 promoter
  • Species
    H. sapiens (human)
  • Mutation
    promoter sequence derived from HepG2 cells
  • Entrez Gene
    RND3 (a.k.a. ARHE, Rho8, RhoE, memB)
  • Tag / Fusion Protein
    • Luciferase (under Rnd3 promoter control) (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer RVprimer3
  • 3′ sequencing primer Luc2-Rev (GCCCTTCTTAATGTTTTTG)
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL4.10-Rnd3 promoter [-1759 +248] was a gift from Violaine Moreau (Addgene plasmid # 86438 ; http://n2t.net/addgene:86438 ; RRID:Addgene_86438)
Related items:
Commonly requested with: