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mouse pGL3-ank3_1-promoter firefly luciferase
(Plasmid #86436)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86436 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL3
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4818
  • Total vector size (bp) 6696
  • Modifications to backbone
    insertion of ank3_1 promoter sequence
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ank3_1 promoter
  • Alt name
    ankyrin-G gene promoter 1
  • Alt name
    MYRT-37 gene promoter 1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1903
  • Mutation
    n/a
  • Promoter ankyrin-3_1
  • Tag / Fusion Protein
    • n/a

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (unknown if destroyed)
  • 3′ cloning site NheI (unknown if destroyed)
  • 5′ sequencing primer RVPrimer3
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

König et al., NF-κB regulates neuronal ankyrin-G via a negative feedback loop, Scientific Reports, 2017

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mouse pGL3-ank3_1-promoter firefly luciferase was a gift from Hans-Georg Koenig & Jochen Prehn (Addgene plasmid # 86436 ; http://n2t.net/addgene:86436 ; RRID:Addgene_86436)
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