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Addgene

p1324 HPV-16 E7
(Plasmid #8643)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8643 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p1318
  • Backbone size w/o insert (bp) 7900
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HPV-16 E7
  • Species
    Human papillomavirus
  • Insert Size (bp)
    334
  • Entrez Gene
    E7 (a.k.a. HpV16gp2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site HIndIII (not destroyed)
  • 5′ sequencing primer na
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See author's map.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p1324 HPV-16 E7 was a gift from Peter Howley (Addgene plasmid # 8643 ; http://n2t.net/addgene:8643 ; RRID:Addgene_8643)

  • For your References section:

    The E6 and E7 genes of the human papillomavirus type 16 together are necessary and sufficient for transformation of primary human keratinocytes. Münger K, Phelps WC, Bubb V, Howley PM, Schlegel R. J Virol. 1989 Oct . 63(10):4417-21. PubMed 2476573
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