pSJ1458 (pFA6-link-mTurquoise2-CaURA3MX)
(Plasmid #86424)

• Purpose: PCR tagging module with yeast codon optimized mTurquoise2
• Depositing Lab: Sue Jaspersen
• Publication: Burns et al Elife. 2015 Sep 15;4. doi: 10.7554/eLife.08586.

Backbone

• Vector backbone: pFA6-link-CaURA3MX
• Backbone size w/o insert (bp): 4200
• Total vector size (bp): 4900
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mTurquoise2
• Species: S. cerevisiae (budding yeast), Synthetic; yeast codon optimized mTurquoise2 for expression in S. cerevisiae
• Insert Size (bp): 716

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AscI (not destroyed)
• 3′ cloning site: PacI (not destroyed)
• 5′ sequencing primer: F5
• 3′ sequencing primer: R3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: cloned into pKT209, a vector from Kurt Thorn

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

use F5/R3 primers