pSJ1726 (pFA6-NATMX-CDC42pr-KAR2SS-GFP1-10)
(Plasmid
#86421)
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PurposeN-terminal PCR tagging cassette for yeast codon optimized split GFP1-10 with CDC42 promoter and KAR2 signal sequence
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 86421 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFA6-NATMX
- Backbone size w/o insert (bp) 4000
- Total vector size (bp) 5263
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Vector typeYeast Expression
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Selectable markersclonNAT
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCDC42pr-KAR2SS-GFP1-10
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SpeciesS. cerevisiae (budding yeast), Synthetic
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Tag
/ Fusion Protein
- GFP1-10 (N terminal on insert)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSJ1726 (pFA6-NATMX-CDC42pr-KAR2SS-GFP1-10) was a gift from Sue Jaspersen (Addgene plasmid # 86421 ; http://n2t.net/addgene:86421 ; RRID:Addgene_86421) -
For your References section:
Analysis of membrane proteins localizing to the inner nuclear envelope in living cells. Smoyer CJ, Katta SS, Gardner JM, Stoltz L, McCroskey S, Bradford WD, McClain M, Smith SE, Slaughter BD, Unruh JR, Jaspersen SL. J Cell Biol. 2016 Nov 21;215(4):575-590. Epub 2016 Nov 9. 10.1083/jcb.201607043 PubMed 27831485
Map uploaded by the depositor.
