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PurposeC-terminal PCR tagging module for yeast codon optimized split GFP1-10
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 86419 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFA6-link-CaURA3MX
- Backbone size w/o insert (bp) 8200
- Total vector size (bp) 8965
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGFP1-10
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SpeciesS. cerevisiae (budding yeast), Synthetic
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
for C-terminal PCR tagging with GFP1-10
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSJ1256 (pFA6-link-yGFP1-10-CaURA3MX) was a gift from Sue Jaspersen (Addgene plasmid # 86419 ; http://n2t.net/addgene:86419 ; RRID:Addgene_86419) -
For your References section:
Analysis of membrane proteins localizing to the inner nuclear envelope in living cells. Smoyer CJ, Katta SS, Gardner JM, Stoltz L, McCroskey S, Bradford WD, McClain M, Smith SE, Slaughter BD, Unruh JR, Jaspersen SL. J Cell Biol. 2016 Nov 21;215(4):575-590. Epub 2016 Nov 9. 10.1083/jcb.201607043 PubMed 27831485