pSJ1256 (pFA6-link-yGFP1-10-CaURA3MX)
(Plasmid #86419)

• Purpose: C-terminal PCR tagging module for yeast codon optimized split GFP1-10
• Depositing Lab: Sue Jaspersen
• Publication: Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590. Epub 2016 Nov 9.

Backbone

• Vector backbone: pFA6-link-CaURA3MX
• Backbone size w/o insert (bp): 8200
• Total vector size (bp): 8965
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GFP1-10
• Species: S. cerevisiae (budding yeast), Synthetic

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

for C-terminal PCR tagging with GFP1-10