pSTAP-seq_fly-tj
(Plasmid #86386)

• Purpose: Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.
• Depositing Lab: Alexander Stark
• Publication: Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.1038/nbt.3739.

Backbone

• Vector backbone: pGL3
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 3426
• Vector type: STAP-seq screening vector

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: D. melanogaster tj enhancer
• Species: D. melanogaster (fly)
• Insert Size (bp): 856

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: RVprimer3

Resource Information

• Supplemental Documents:
  • pSTAP-seq_fly-tj.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

to consruct the STAP-seq screening vector from pGL3 Promoter we replaced the sequence between the BglII and FseI sites with the following sequence, containing a ccdB suicide gene next to the Chloramphenicole resistance gene flanked by homology arms (used for cloning the candidates during library generation), an intron (mhc16), an ORF (truncated sgGFP, Qbiogene, Inc)