pSws1-nfsB-CFP
(Plasmid #86375)

• Purpose: for zebrafish transgenesis, expression of nfsB-CFP, also expresses EGFP
• Depositing Lab: Rachel Wong
• Publication: Yoshimatsu et al Nat Commun. 2016 Feb 3;7:10590. doi: 10.1038/ncomms10590.

Backbone

• Vector backbone: pDestTol2pA
• Vector type: zebrafish

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: nitroreductase
• Alt name: nfsB
• Species: bacteria
• Entrez Gene: nfsB (a.k.a. b0578, ECK0570, JW0567, dprA, nfnB, nfsI, ntr)
• Tag / Fusion Protein:
  • ECFP (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: SP6
• 3′ sequencing primer: EGFP-N

Resource Information

• Supplemental Documents:
  • pSws1-nfsB-CFP.str

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • National Institute of Genetics Limited Use Label License for Tol2 System
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene's next-generation sequencing (NGS) found an R29M mutation in backbone element medaka tyrosinase. The depositing lab has not determined the functionality of this tyrosinase variant.