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Purposeexpression of nfsB-CFP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 86374 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepME
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Vector typezebrafish
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namenitroreductase
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Alt namenfsB
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Speciesbacteria
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Tag
/ Fusion Protein
- ECFP (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer SP6
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pME-nfsB-CFP was a gift from Rachel Wong (Addgene plasmid # 86374 ; http://n2t.net/addgene:86374 ; RRID:Addgene_86374) -
For your References section:
Presynaptic partner selection during retinal circuit reassembly varies with timing of neuronal regeneration in vivo. Yoshimatsu T, D'Orazi FD, Gamlin CR, Suzuki SC, Suli A, Kimelman D, Raible DW, Wong RO. Nat Commun. 2016 Feb 3;7:10590. doi: 10.1038/ncomms10590. 10.1038/ncomms10590 PubMed 26838932