pUAS-MmTFP13xmyc
(Plasmid
#86371)
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Purposemembrane targeted Tfp1 expression in zebrafish
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 86371 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUAS
- Total vector size (bp) 4682
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Vector typezebrafish
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemTfp1
- Promoter UAS
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Tags
/ Fusion Proteins
- GAP43 membrane targeting (N terminal on insert)
- 3xMyc (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pBR322ori-F (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUAS-MmTFP13xmyc was a gift from Rachel Wong (Addgene plasmid # 86371 ; http://n2t.net/addgene:86371 ; RRID:Addgene_86371) -
For your References section:
Presynaptic partner selection during retinal circuit reassembly varies with timing of neuronal regeneration in vivo. Yoshimatsu T, D'Orazi FD, Gamlin CR, Suzuki SC, Suli A, Kimelman D, Raible DW, Wong RO. Nat Commun. 2016 Feb 3;7:10590. doi: 10.1038/ncomms10590. 10.1038/ncomms10590 PubMed 26838932