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Addgene

YEplac181-TYS1
(Plasmid #8629)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8629 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    YEplac181
  • Backbone size w/o insert (bp) 5741
  • Vector type
    Bacterial Expression, Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TYS1
  • Alt name
    TTS1
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2138
  • Mutation
    Wild type
  • Entrez Gene
    TYS1 (a.k.a. YGR185C, TTS1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PstI (not destroyed)
  • 3′ cloning site SacI (not destroyed)
  • 5′ sequencing primer M13 reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    YEplac181-TYS1 was a gift from Jeff Strathern (Addgene plasmid # 8629 ; http://n2t.net/addgene:8629 ; RRID:Addgene_8629)

  • For your References section:

    Genetic interactions of DST1 in Saccharomyces cerevisiae suggest a role of TFIIS in the initiation-elongation transition. Malagon F, Tong AH, Shafer BK, Strathern JN. Genetics 2004 Mar;166(3):1215-27. 10.1534/genetics.166.3.1215 PubMed 15082542
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