pTol-Promoter:NLS-tdMCP-eGFP
(Plasmid #86248)

• Purpose: For generating transgenic lines that ubiquitously expressing the fluorescent MCP reporter
• Depositing Lab: Florence Marlow
• Publication: Campbell et al Development. 2015 Apr 1;142(7):1368-74. doi: 10.1242/dev.118968. Epub 2015 Mar 10.

Backbone

• Vector backbone: pTolDestR4-R2pA
• Backbone manufacturer: Villefranc et al., 2007
• Modifications to backbone: The βactin (Higashijima et al., 1997) and hsp70l (Halloran et al., 2000) promoter 5′ entry clones (p5E) were previously described (Kwan et al., 2007). Appropriate p5E and pME plasmids were recombined with pTolDestR4-R2pA (Villefranc et al., 2007) following the manufacturer’s instructions (Invitrogen) to make pTolBetaActin: NLS-tdMCP-eGFP.
• Vector type: Used with transposase to make transient or stable transgenic zebrafish lines

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: beta Actin promoter:NLS-tdMCPeGFP
• Tag / Fusion Protein:
  • NLS-tdMCP-eGFP (C terminal on insert)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry