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pME-NLS-tdMCP-eGFP
(Plasmid #86243)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86243 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR8/GW/TOPO (K250020, Invitrogen)
  • Backbone manufacturer
    Invitrogen
  • Modifications to backbone
    NLS-HA-tdMCP-GFP and NLS-HA-tdMCP-tagRFP were amplified by PCR from phage-UBC-NLS-HA-tdMCP-GFP (Addgene, 40649) (Wu et al., 2012) and phage-UBC-NLS-HA-tdMCP-tagRFP (Wu et al., 2012) using NLS-HA-tdMCP-FP-F+R primers. To add flanking attL1 and attL2 sites (Hartley et al., 2000; Walhout et al., 2000), PCR fragments were TOPO cloned into pCR8/GW/TOPO (K250020, Invitrogen) to make pME-NLS-tdMCP-eGFP.
  • Vector type
    Unspecified
  • Tag / Fusion Protein
    • NLS-tdMCP-eGFP (C terminal on insert)

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pME-NLS-tdMCP-eGFP was a gift from Florence Marlow (Addgene plasmid # 86243 ; http://n2t.net/addgene:86243 ; RRID:Addgene_86243)

  • For your References section:

    Dynamic visualization of transcription and RNA subcellular localization in zebrafish. Campbell PD, Chao JA, Singer RH, Marlow FL. Development. 2015 Apr 1;142(7):1368-74. doi: 10.1242/dev.118968. Epub 2015 Mar 10. 10.1242/dev.118968 PubMed 25758462
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