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Addgene

pME-mCherry-24xMBS
(Plasmid #86242)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86242 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PCR8/GW/TOPO (K250020, Invitrogen)
  • Backbone manufacturer
    Invitrogen
  • Modifications to backbone
    pME-mCherry-24xMBS was created by amplifying mCherry with flanking BamHI sites by PCR from p3E-mCherry (Kwan et al., 2007) using p3E-mCherry-BamHI-F+R primers, then ligating into pME-24xMBS
  • Vector type
    Unspecified
  • Tag / Fusion Protein
    • mCherry-24xMBS (C terminal on insert)

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note: due the the repetitive nature of the cassette, Addgene QC did not verify the presence of all 24x MBS; digest results indicate all sites are present based on size.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pME-mCherry-24xMBS was a gift from Florence Marlow (Addgene plasmid # 86242 ; http://n2t.net/addgene:86242 ; RRID:Addgene_86242)

  • For your References section:

    Dynamic visualization of transcription and RNA subcellular localization in zebrafish. Campbell PD, Chao JA, Singer RH, Marlow FL. Development. 2015 Apr 1;142(7):1368-74. doi: 10.1242/dev.118968. Epub 2015 Mar 10. 10.1242/dev.118968 PubMed 25758462
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