pLV-PLXNB2-mRBD
(Plasmid #86240)

• Purpose: Lentivirus for expression of human Plexin-B2 with mutant RBD domain
• Depositing Lab: Roland Friedel
• Publication: Huang et al Commun Biol. 2021 Jan 29;4(1):145. doi: 10.1038/s42003-021-01667-4.

Backbone

• Vector backbone: pLENTI PGK Neo DEST ADDGENE #19067
• Backbone manufacturer: Kaufman laboratory, University of Massachusetts
• Backbone size w/o insert (bp): 9778
• Total vector size (bp): 13669
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Plexin-B2
• Alt name: PLXNB2
• Species: H. sapiens (human)
• Mutation: PLXNB2 with point mutations of RBD domain
• Entrez Gene: PLXNB2 (a.k.a. MM1, Nbla00445, PLEXB2, dJ402G11.3)
• Promoter: hPGK

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: PGK-F

Resource Information

• Supplemental Documents:
  • Genbank file
  • Overview of LV-PLXNB2 constructs
• A portion of this plasmid was derived from a plasmid made by: PLXNB2 from Gateway entry vector pENTR223-PLXNB2, The ORFeome Collaboration

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry