YEplac181-DST1
(Plasmid
#8624)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 8624 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneYEplac181
- Backbone size w/o insert (bp) 5741
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Vector typeBacterial Expression, Yeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDST1
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Alt namePPR2
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)1800
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MutationWild type
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Entrez GeneDST1 (a.k.a. YGL043W, PPR2)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site SalI (destroyed during cloning)
- 5′ sequencing primer M13 reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
YEplac181-DST1 was a gift from Jeff Strathern (Addgene plasmid # 8624 ; http://n2t.net/addgene:8624 ; RRID:Addgene_8624) -
For your References section:
Genetic interactions of DST1 in Saccharomyces cerevisiae suggest a role of TFIIS in the initiation-elongation transition. Malagon F, Tong AH, Shafer BK, Strathern JN. Genetics 2004 Mar;166(3):1215-27. 10.1534/genetics.166.3.1215 PubMed 15082542