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PurposeExpress fluorescent calcium indicator GCaMP6fu in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 86235 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-N1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 3945
- Total vector size (bp) 5308
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGCaMP6fu
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SpeciesR. norvegicus (rat); A. victoria (jellyfish)
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Insert Size (bp)1323
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MutationW43Y D395A
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer EGFP-N (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byA portion of this plasmid was derived from a plasmid made by Douglas Kim
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-GCaMP6fu was a gift from Katalin Torok (Addgene plasmid # 86235 ; http://n2t.net/addgene:86235 ; RRID:Addgene_86235) -
For your References section:
Design and mechanistic insight into ultrafast calcium indicators for monitoring intracellular calcium dynamics. Helassa N, Podor B, Fine A, Torok K. Sci Rep. 2016 Dec 6;6:38276. doi: 10.1038/srep38276. 10.1038/srep38276 PubMed 27922063
