pRS415-SOH1
(Plasmid
#8622)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8622 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRS415
- Backbone size w/o insert (bp) 6021
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Vector typeBacterial Expression, Yeast Expression
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsNotice that is low copy in S. cerevisiae buth High copy for E. coli
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameSOH1
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)1077
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MutationWild type
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Entrez GeneSOH1 (a.k.a. YGL127C, MED31)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRS415-SOH1 was a gift from Jeff Strathern (Addgene plasmid # 8622 ; http://n2t.net/addgene:8622 ; RRID:Addgene_8622) -
For your References section:
Genetic interactions of DST1 in Saccharomyces cerevisiae suggest a role of TFIIS in the initiation-elongation transition. Malagon F, Tong AH, Shafer BK, Strathern JN. Genetics 2004 Mar;166(3):1215-27. 10.1534/genetics.166.3.1215 PubMed 15082542
