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Addgene

pBbA5k-Maqu_3494
(Plasmid #86201)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86201 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBbA5k
  • Total vector size (bp) 7924
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Efflux pump
  • Insert Size (bp)
    4254
  • Promoter ATCGTTTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAAT

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GGATCCAAACTCGAGTAAGG
  • 3′ sequencing primer GATCTTTTAAGAAGGAGATATACAT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid carries G179V in Maqu_3494 compared to the genomic reference sequence. This mutation does not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBbA5k-Maqu_3494 was a gift from Mary Dunlop (Addgene plasmid # 86201 ; http://n2t.net/addgene:86201 ; RRID:Addgene_86201)

  • For your References section:

    Trade-Offs in Improving Biofuel Tolerance Using Combinations of Efflux Pumps. Turner WJ, Dunlop MJ. ACS Synth Biol. 2015 Oct 16;4(10):1056-63. doi: 10.1021/sb500307w. Epub 2014 Dec 12. 10.1021/sb500307w PubMed 25496359
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