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Addgene

pMT1121
(Plasmid #8620)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8620 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Backbone size w/o insert (bp) 2641
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Requires strain with the lacI^Q gene (such as DH5alpha, XL1-blue, SURE or HB107)
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ribonuclease Sa, barstar
  • Species
    Streptomyces aureofaciens, Strain BMK
  • Insert Size (bp)
    973
  • GenBank ID
    U39467

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer M13F
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    J. Sevcik Laboratory of Molecular Biology Slovak Academy of Science, Bratislava, Slovakia

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

As Pmt1100 but with synthetic gene for ribonuclease Sa based on its amino acid sequence. Ribonuclease Sa from S. aureofaciens strain CCM 3239. 195-486: Structural gene for mature ribonuclease Sa. Genbank #U39467.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMT1121 was a gift from Robert Hartley (Addgene plasmid # 8620 ; http://n2t.net/addgene:8620 ; RRID:Addgene_8620)

  • For your References section:

    Barstar inhibits extracellular ribonucleases of Streptomyces and allows their production from recombinant genes. Hartley RW, Both V, Hebert EJ, Homerova D, Jucovic M, Nazarov V, Rybajlak I and Sevcik J. Protein Peptide Letters 1996 3:225-231
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