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Addgene

pMT1101
(Plasmid #8619)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8619 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Backbone size w/o insert (bp) 2641
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ribonuclease Sa2, barstar
  • Species
    Streptomyces aureofaciens, Strain R8/26
  • Insert Size (bp)
    976
  • GenBank ID
    M88615

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer M13F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

As pMT1100 but with gene for ribonuclease Sa2. Ribonuclease gene Sa2 from S. aureofaciens strain R8/26. 195-489: Structural gene for mature ribonuclease Sa2. Genbank #M88615.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMT1101 was a gift from Robert Hartley (Addgene plasmid # 8619 ; http://n2t.net/addgene:8619 ; RRID:Addgene_8619)

  • For your References section:

    Barstar inhibits extracellular ribonucleases of Streptomyces and allows their production from recombinant genes. Hartley RW, Both V, Hebert EJ, Homerova D, Jucovic M, Nazarov V, Rybajlak I and Sevcik J. Protein Peptide Letters 1996 3:225-231
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