pMT1100
(Plasmid #8618)

• Depositing Lab: Robert Hartley
• Publication: Hartley et al Protein Peptide Letters 1996 3:225-231

Backbone

• Vector backbone: pUC19
• Backbone size w/o insert (bp): 2641
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Requires strain with the lacI^Q gene (such as DH5alpha, XL1-blue, SURE or HB107)
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ribonuclease Sa3, barstar
• Species: Streptomyces aureofaciens, Strain BMK
• Insert Size (bp): 982
• Mutation: In addition to the substitution of the barnase gene of pMT316 with that of RNase Sa3, a 121 base segment between the RNase gene and that of barstar has been replaced by a 68 base sequence including a synthetic translation terminator.
• GenBank ID: M82920
• Tag / Fusion Protein:
  • phoA signal sequence to RNase Sa3 (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: M13F
• 3′ sequencing primer: M13R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: J. Sevcik Laboratory of Molecular Biology Slovak Academy of Science, Bratislava, Slovakia

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

As pMT413 with the mature barnase structural gene and its
3' sequence replaced by that of ribonuclease Sa3 and a synthetic transcription
terminator (AGCCC GCCTA ATGAG CGGGC TTTTT TTTTG). Ribonuclease Sa3 gene from Streptomyces aureofaciens strain BMK. 195-495: Structural gene for mature ribonuclease Sa3. Genbank #M82920.
540-656 barstar 3