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Addgene

pstb-LAFR5-exoY
(Plasmid #86170)

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Item Catalog # Description Quantity Price (USD)
Plasmid 86170 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pstb-LAFR5
  • Backbone manufacturer
    Kay Jones
  • Total vector size (bp) 22700
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    exoY
  • Species
    S. meliloti
  • Promoter Smb21651

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PstI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer M13F
  • 3′ sequencing primer M13R
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pstb-LAFR5-exoY was a gift from Kay Jones (Addgene plasmid # 86170 ; http://n2t.net/addgene:86170 ; RRID:Addgene_86170)

  • For your References section:

    Increased production of the exopolysaccharide succinoglycan enhances Sinorhizobium meliloti 1021 symbiosis with the host plant Medicago truncatula. Jones KM. J Bacteriol. 2012 Aug;194(16):4322-31. doi: 10.1128/JB.00751-12. Epub 2012 Jun 8. 10.1128/JB.00751-12 PubMed 22685282
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