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Addgene

HOXA10 (human) bluescript
(Plasmid #8617)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8617 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript II SK(+)
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 2950
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HOXA10
  • Alt name
    HOX1H
  • Alt name
    HOX1.8
  • Alt name
    PL1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1820
  • Mutation
    improved Ribosomal binding site (NcoI site) at ATG start codon; designed for in vitro transcription/translation without FLAG tag
  • Entrez Gene
    HOXA10 (a.k.a. HOX1, HOX1.8, HOX1H, PL)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HOXA10 (human) bluescript was a gift from Corey Largman (Addgene plasmid # 8617 ; http://n2t.net/addgene:8617 ; RRID:Addgene_8617)

  • For your References section:

    A human Hox 1 homeobox gene exhibits myeloid-specific expression of alternative transcripts in human hematopoietic cells. Lowney P, Corral J, Detmer K, LeBeau MM, Deaven L, Lawrence HJ, Largman C. Nucleic Acids Res 1991 Jun 25;19(12):3443-9. 10.1093/nar/19.12.3443 PubMed 1676505
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