p35S::AtSOBIR1-YFPn
(Plasmid
#86165)
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PurposeSOBIR1 split YFP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 86165 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAM-PAT-GWPro35S
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSOBIR1
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SpeciesA. thaliana (mustard weed)
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Entrez GeneSOBIR1 (a.k.a. AT2G31880, EVERSHED, EVR, F20M17.8, F20M17_8, SUPPRESSOR OF BIR1 1)
- Promoter 35S
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Tag
/ Fusion Protein
- YFPn (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer 35S promoter (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p35S::AtSOBIR1-YFPn was a gift from Silke Robatzek (Addgene plasmid # 86165 ; http://n2t.net/addgene:86165 ; RRID:Addgene_86165) -
For your References section:
Avr4 promotes Cf-4 receptor-like protein association with the BAK1/SERK3 receptor-like kinase to initiate receptor endocytosis and plant immunity. Postma J, Liebrand TW, Bi G, Evrard A, Bye RR, Mbengue M, Kuhn H, Joosten MH, Robatzek S. New Phytol. 2016 Apr;210(2):627-42. doi: 10.1111/nph.13802. Epub 2016 Jan 14. 10.1111/nph.13802 PubMed 26765243