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Addgene

pCAMBIA-FLS2-myc-mCherry
(Plasmid #86159)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86159 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAMBIA2300
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FLS2
  • Species
    A. thaliana (mustard weed)
  • Entrez Gene
    FLS2 (a.k.a. AT5G46330, FLAGELLIN-SENSITIVE 2, MPL12.13, MPL12.8, MPL12_13)
  • Tags / Fusion Proteins
    • myc (C terminal on insert)
    • mCherry (C terminal on insert)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAMBIA-FLS2-myc-mCherry was a gift from Silke Robatzek (Addgene plasmid # 86159 ; http://n2t.net/addgene:86159 ; RRID:Addgene_86159)

  • For your References section:

    Clathrin-dependent endocytosis is required for immunity mediated by pattern recognition receptor kinases. Mbengue M, Bourdais G, Gervasi F, Beck M, Zhou J, Spallek T, Bartels S, Boller T, Ueda T, Kuhn H, Robatzek S. Proc Natl Acad Sci U S A. 2016 Sep 27;113(39):11034-9. doi: 10.1073/pnas.1606004113. Epub 2016 Sep 20. 10.1073/pnas.1606004113 PubMed 27651493
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