pCAMBIA-FLS2prom::FLS2-3xmyc
(Plasmid
#86158)
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PurposeFLS2 expression
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 86158 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCAMBIA2300
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFLS2
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SpeciesA. thaliana (mustard weed)
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Entrez GeneFLS2 (a.k.a. AT5G46330, FLAGELLIN-SENSITIVE 2, MPL12.13, MPL12.8, MPL12_13)
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Tag
/ Fusion Protein
- 3xmyc (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13R
- 3′ sequencing primer M13F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAMBIA-FLS2prom::FLS2-3xmyc was a gift from Silke Robatzek (Addgene plasmid # 86158 ; http://n2t.net/addgene:86158 ; RRID:Addgene_86158) -
For your References section:
Ligand-induced endocytosis of the pattern recognition receptor FLS2 in Arabidopsis. Robatzek S, Chinchilla D, Boller T. Genes Dev. 2006 Mar 1;20(5):537-42. 10.1101/gad.366506 PubMed 16510871
