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Addgene

pSaI20
(Plasmid #8615)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8615 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTrc99A
  • Backbone size w/o insert (bp) 4125
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ribonuclease inhibitor SaI20
  • Species
    Streptomyces aureofaciens, Strain R8/26
  • Insert Size (bp)
    312
  • Mutation
    N terminal threnine changed to alanine
  • GenBank ID
    AY374310

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer na
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Production plasmid for ribonuclease inhibitor
and barstar homolog SaI20 from S. aureofaciens. Vector as
for pSaI14.
Structural gene from S. aureofaciens strain R8/26. N-terminal threonine
of wild type changed to alanine.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSaI20 was a gift from Robert Hartley (Addgene plasmid # 8615 ; http://n2t.net/addgene:8615 ; RRID:Addgene_8615)

  • For your References section:

    Isolation and purification of two novel streptomycete RNase inhibitors, SaI14 and SaI20, and cloning, sequencing, and expression in Escherichia coli of the gene coding for SaI14. Krajcikova D, Hartley RW, Sevcik J. J Bacteriol 1998 Mar;180(6):1582-5. PubMed 9515932
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