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Addgene

FLAG-mPar6a K19A
(Plasmid #86149)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86149 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MSCV -PGK-Puro-IRES-GFP
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Par6
  • Alt name
    Par6 alpha
  • Alt name
    Par6c
  • Species
    M. musculus (mouse)
  • Mutation
    Lysine to Alanine mutation K19A
  • Entrez Gene
    Pard6a (a.k.a. 0710008C04Rik, 2610010A15Rik, PAR-6A, PAR6alpha, Par-6, Par6, Par6c, TAX40, Tip-40)
  • Promoter LTR
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hpa1 site (unknown if destroyed)
  • 3′ cloning site Hpa1 site (unknown if destroyed)
  • 5′ sequencing primer MSCV-F
  • 3′ sequencing primer MSCV-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLAG-mPar6a K19A was a gift from Senthil Muthuswamy (Addgene plasmid # 86149 ; http://n2t.net/addgene:86149 ; RRID:Addgene_86149)

  • For your References section:

    The polarity protein Par6 induces cell proliferation and is overexpressed in breast cancer. Nolan ME, Aranda V, Lee S, Lakshmi B, Basu S, Allred DC, Muthuswamy SK.. Cancer Res. 2008 Oct 15;68(20):8201-9. 10.1158/0008-5472.CAN-07-6567 PubMed 18922891
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