pcDNAHygro(+)LAMP2AS
(Plasmid #86146)

• Purpose: Anti sense for LAMP2
• Depositing Lab: Janice Blum
• Publication: Zhou et al Immunity. 2005 May;22(5):571-81.

Backbone

• Vector backbone: pcDNAHygro(+)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5000
• Total vector size (bp): 5658
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB 5-alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: LAMP2a
• Species: H. sapiens (human)
• Insert Size (bp): 528
• Mutation: contains first 525 bases of LAMP cDNA
• Entrez Gene: LAMP2 (a.k.a. CD107b, DND, LAMP-2, LAMPB, LGP-96, LGP110)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Hind III (unknown if destroyed)
• 3′ cloning site: Kpn1 (unknown if destroyed)
• 5′ sequencing primer: M13 forward
• 3′ sequencing primer: M13 reverse

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Insert in inverted orientation. Knocks down LAMP2A and 2B.