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Addgene

pcDNAHygro(+)LAMP2AS
(Plasmid #86146)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86146 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNAHygro(+)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 5658
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB 5-alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    LAMP2a
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    528
  • Mutation
    contains first 525 bases of LAMP cDNA
  • Entrez Gene
    LAMP2 (a.k.a. CD107b, DND, LAMP-2, LAMPB, LGP-96, LGP110)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hind III (unknown if destroyed)
  • 3′ cloning site Kpn1 (unknown if destroyed)
  • 5′ sequencing primer M13 forward
  • 3′ sequencing primer M13 reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert in inverted orientation. Knocks down LAMP2A and 2B.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNAHygro(+)LAMP2AS was a gift from Janice Blum (Addgene plasmid # 86146 ; http://n2t.net/addgene:86146 ; RRID:Addgene_86146)

  • For your References section:

    Lamp-2a facilitates MHC class II presentation of cytoplasmic antigens. Zhou D, Li P, Lin Y, Lott JM, Hislop AD, Canaday DH, Brutkiewicz RR, Blum JS. Immunity. 2005 May;22(5):571-81. 10.1016/j.immuni.2005.03.009 PubMed 15894275
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