pSaI14
(Plasmid
#8614)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8614 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepTrc99A
- Backbone size w/o insert (bp) 4125
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRibonuclease inhibitor SaI14
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SpeciesStreptomyces aureofaciens, Strain R8/26
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Insert Size (bp)381
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MutationN terminal threonine changed to alanine
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GenBank IDAF020428
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Production plasmid for ribonuclease inhibitor
and barstar homolog SaI14 from S. aureofaciens. The gene is
carried between the Nco1 and HindIII sites of pTrc99A (Genbank
#M22744).
Structural gene from S. aureofaciens strain R8/26. N-terminal threonine
of the wild type changed to alanine.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSaI14 was a gift from Robert Hartley (Addgene plasmid # 8614 ; http://n2t.net/addgene:8614 ; RRID:Addgene_8614) -
For your References section:
Isolation and purification of two novel streptomycete RNase inhibitors, SaI14 and SaI20, and cloning, sequencing, and expression in Escherichia coli of the gene coding for SaI14. Krajcikova D, Hartley RW, Sevcik J. J Bacteriol 1998 Mar;180(6):1582-5. PubMed 9515932