Skip to main content
Addgene

pSaI14
(Plasmid #8614)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8614 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pTrc99A
  • Backbone size w/o insert (bp) 4125
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ribonuclease inhibitor SaI14
  • Species
    Streptomyces aureofaciens, Strain R8/26
  • Insert Size (bp)
    381
  • Mutation
    N terminal threonine changed to alanine
  • GenBank ID
    AF020428

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer na
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Production plasmid for ribonuclease inhibitor
and barstar homolog SaI14 from S. aureofaciens. The gene is
carried between the Nco1 and HindIII sites of pTrc99A (Genbank
#M22744).
Structural gene from S. aureofaciens strain R8/26. N-terminal threonine
of the wild type changed to alanine.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSaI14 was a gift from Robert Hartley (Addgene plasmid # 8614 ; http://n2t.net/addgene:8614 ; RRID:Addgene_8614)

  • For your References section:

    Isolation and purification of two novel streptomycete RNase inhibitors, SaI14 and SaI20, and cloning, sequencing, and expression in Escherichia coli of the gene coding for SaI14. Krajcikova D, Hartley RW, Sevcik J. J Bacteriol 1998 Mar;180(6):1582-5. PubMed 9515932
Related items:
Commonly requested with: